Preparation of Dialyzable Histocompatibility Antigen from BALB/c Mice

Preparation of dialyzable histocompatibility antigen from BALB-c mice (cell-membrane fragments-proteolytic digestion-detergent solubilization).

Histocompatibility antigens solubilized from cell-membrane fragments of BALB/c mouse spleen and liver, by Triton X-100 and butyl alcohol, were subjected to digestion by proteolytic enzymes in an effort to obtain smaller molecular species that retained antigenic activity. Digestion with both trypsin and papain yielded two antigens of smaller molecular weights that retained the specificity of BAL...

Preparation and characterization of a soluble precipitating antigen from African horsesickness virus propagated in cell cultures

Preparation of Core Antigen

Hepatitis B core antigen (HBcAg) was prepared from human liver tissue and used in an immunoelectro-osmophoresis screening test to detect antibody to HBcAg (anti-HBc) in patients with evidence of liver disease and in blood donors. With the exception of two immunosuppressed HBsAg carriers, anti-HBc was found in all cases of hepatitis B infection even when HBsAg was detectable only by radioimmunoa...

The Immunogenicity of a New Human Minor Histocompatibility Antigen Results from Differential Antigen Processing

Minor histocompatibility antigens (mHAgs) present a significant impediment to organ and bone marrow transplantation between HLA-identical donor and recipient pairs. Here we report the identification of a new HLA-A*0201-restricted mHAg, HA-8. Designation of this mHAg as HA-8 is based on the nomenclature of Goulmy (Goulmy, E. 1996. Curr. Opin. Immunol. 8:75-81). This peptide, RTLDKVLEV, is derive...

Preparation of Trichomonal Antigen

An enzyme immunoassay (EIA) was developed for the detection of Trichomonas vaginalis antigen in vaginal swabs. Four hundred and eighty two women attending a sexually transmitted disease (STD) clinic were tested; 44 (9 1 %) were positive by culture, 32 (6-6%) were positive by wet film examination, and 54 (11 2%) were considered to be positive for trichomonal antigen by EIA. Taking culture as the...